In ordinary terms a cytometer is an average sized equipment with a specific volume which is employed in scientific examination of cellular materials. The most current devices can easily assess a large number of cells per second . These devices are able to easily differentiate and define cellular elements as per their attributes.
One can easily confuse a this equipment for a microscope. This is because the two devices are related in a number of ways. However, they are different from microscopes in the sense that they are able to regulate and quantify the parameters being set. Their working is also based on laser technology which is not so common with many microscopes.
Combinations of illuminations of extended wavelength emerging from the particles are then collected by detectors which analyze the variations in their intensities. The analysis is then used to develop numerous kinds of data concerning the actual physical and chemical design of every single particle. This means that every element in a cell scatters light differently.
When carrying out the examination of a specimen, the light is directed on the sheath fluid that is strategically located in the machine. This light is usually at a regulated wavelength since the desired results can only occur at specific wavelengths. After striking the surface of the fluid this light bounces back as a spectrum which is picked up by various detectors that are strategically placed inside the device. This is what is taken in as the input data for the machine.
Florescent inorganic elements contained in the particles being examined block and reflect the light from the source at extended wavelengths compared to the original rays. These reflected rays of longer wavelengths are the ones which are analyzed to come up with reliable data about the chemical and physical composition of all particles being examined. It should be noted that every element inside a cell reflects light distinctively.
An extensive evaluation of these rays is then carried out by the use of a computer which is connected to the cytometer via several cables. Specific computer applications are employed during this process of evaluation. These software have the ability of accepting inputs, processing it and delivering very accurate and properly displayed reports about the particles being studied.
Histograms and graphs are among the few ways in which data which is processed by computers can be presented after analysis. This is how users of these gadgets are able to adequately determine various readings that lie between the recorded values. Also, digital images of specimen can be obtained as outputs once processing is complete.
You can find the application of a cytometer in quite a number of fields today. Some of these fields include: marine biology, protein engineering, molecular biology and pathology just to name a few. Marine biologist use this gadget to determine the various attributes of photosynthetic plankton in an effort to characterize their abundance in a given habitat.
One can easily confuse a this equipment for a microscope. This is because the two devices are related in a number of ways. However, they are different from microscopes in the sense that they are able to regulate and quantify the parameters being set. Their working is also based on laser technology which is not so common with many microscopes.
Combinations of illuminations of extended wavelength emerging from the particles are then collected by detectors which analyze the variations in their intensities. The analysis is then used to develop numerous kinds of data concerning the actual physical and chemical design of every single particle. This means that every element in a cell scatters light differently.
When carrying out the examination of a specimen, the light is directed on the sheath fluid that is strategically located in the machine. This light is usually at a regulated wavelength since the desired results can only occur at specific wavelengths. After striking the surface of the fluid this light bounces back as a spectrum which is picked up by various detectors that are strategically placed inside the device. This is what is taken in as the input data for the machine.
Florescent inorganic elements contained in the particles being examined block and reflect the light from the source at extended wavelengths compared to the original rays. These reflected rays of longer wavelengths are the ones which are analyzed to come up with reliable data about the chemical and physical composition of all particles being examined. It should be noted that every element inside a cell reflects light distinctively.
An extensive evaluation of these rays is then carried out by the use of a computer which is connected to the cytometer via several cables. Specific computer applications are employed during this process of evaluation. These software have the ability of accepting inputs, processing it and delivering very accurate and properly displayed reports about the particles being studied.
Histograms and graphs are among the few ways in which data which is processed by computers can be presented after analysis. This is how users of these gadgets are able to adequately determine various readings that lie between the recorded values. Also, digital images of specimen can be obtained as outputs once processing is complete.
You can find the application of a cytometer in quite a number of fields today. Some of these fields include: marine biology, protein engineering, molecular biology and pathology just to name a few. Marine biologist use this gadget to determine the various attributes of photosynthetic plankton in an effort to characterize their abundance in a given habitat.